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Wheat, as one of the main cereal crops, is the primary source of human dietary nutrition. Biological breeding methods can increase the content of vitamin E in wheat grains, especially the α-tocopherol. This study Agrobacterium-mediated transferred AtHPT and AtTC genes into hexaploid wheat Fielder, and selected the lines with high expression of AtHPT and AtTC genes in grains through qRT-PCR experiments. HPLC was performed on their tocopherol content, and the plant height, grain length, grain width, thousand grain weight, spikelet number, and grain per spike were determined. The results showed that transgenic wheat overexpression lines of AtTC gene showed no significant difference in the content of δ-tocopherol compared to Fielder, the content of α-tocopherol was significantly increased in transgenic wheat grains, β-tocopherol was no detected, and the agronomic traits of wheat were significantly increased in spike length, number of spikelets, and number of grains per spike in the offspring lines. While the transgenic wheat lines overexpressing the AtHPT gene showed no significant difference in β- or δ-tocopherols content compared to Fielder, α-tocopherol was no detected. γ-tocopherol was no detected in transgenic wheats of AtHPT and AtTC. The above results indicate that overexpression of AtTC can significumtly increase the content of α-taopheral in wheat grains without apparent negative effects on wheat yield traits.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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