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Outline
Stomata affects transpiration and photosynthesis in plants by controlling the gas exchange between the internal and external environments of leaves. It plays an important role in plant growth and development, and adaptation to environmental changes. To explore the potential function of TaMUTE, a gene related to wheat stomatal development, in plant growth and development, was cloned from the common wheat and analyzed for its sequence characteristics. The expression pattern of TaMUTE gene was analyzed under drought and abscisic acid (ABA) stresses by qRT-PCR. The TaMUTE transgenic plants were obtained by transgenic technology and analyzed for their phenotype. The results showed that three TaMUTE homologs gene were cloned from common wheat, which were named TaMUTE2A, TaMUTE2B and TaMUTE2D. The open reading frame (ORF) of the wheat TaMUTE2A gene is 651 bp, encoding 216 amino acids; the ORF of the TaMUTE2B gene is 717 bp, encoding 238 amino acids; and the ORF of the TaMUTE2D gene is 648 bp, encoding 215 amino acids. Three MUTE proteins belong to the bHLH (basic helix-loop-helix) family, which are all hydrophobic and labile proteins. Their secondary structure is mainly composed of α-helix, extended chain, β-turn and irregular recoil. Phylogenetic analysis revealed that TaMUTE is more closely related to the barley MUTE. The expression of the TaMUTE was significantly enhanced after 4 h under ABA treatment. While the expression of the TaMUTE gene did not change significantly under the drought treatment conditions. Overexpression of TaMUTE genes resulted in short stature, development retardation and increased stomatal density in transgenic plant.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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