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Outline
In order to investigate the control efficacy of the combination of Bacillus methylotrophicus Lw-6 and chemical fungicides against wheat stripe rust, Puccinia striiformis f. sp. tritici (Pst) urediniospores were used as the target fungus, and strain Lw-6 was screened through antagonistic tests in vitro. The chemical fungicide used in combination with Lw-6 was selected by compatibility testing, and the optimal concentration was selected by greenhouse pot tests. Finally, the control and growth promotion effects were determined by greenhouse pot tests and field test. The results showed that diniconazole had the best compatibility with strain Lw-6, and the combined application of 128 mg·L-1 Di and Lw-6 fermentation liquid achieved 92.78% and 86.22% control efficacy against wheat stripe rust in pot experiments and field trials, respectively, which were higher than the controlling effects of using individual diniconazole or Lw-6. In addition, the 1 000-grain weight and yield of wheat were significantly increased after compound treatment, and the contribution rate of plant protection of 6 500 mg·L-1 Lw-6 powder combined with 128 mg·L-1 diniconazole was 24.98%. In summary, the combination of Lw-6 and diniconazole can effectively control wheat stripe rust, and it has promising application potential.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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