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DNA Fingerprint Construction and Genetic Diversity Analysis of Wheat Germplasms in Pingdingshan Region
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Erwei WANG1, Aichu MA1, Jiansheng WANG2, Haohao YANG2, Junfeng WANG3, Yamin HUANG1, Ruofei GENG1, Qinqun JIANG1
Journal of Triticeae Crops | 2025, 45(10) : 1342 - 1350
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Journal of Triticeae Crops | 2025, 45(10): 1342-1350
• Genetics & Breedin •
DNA Fingerprint Construction and Genetic Diversity Analysis of Wheat Germplasms in Pingdingshan Region
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Erwei WANG1, Aichu MA1, Jiansheng WANG2, Haohao YANG2, Junfeng WANG3, Yamin HUANG1, Ruofei GENG1, Qinqun JIANG1
Affiliations
  • 1.Pingdingshan Academy of Agricultural Science, Pingdingshan, Henan 467001, China
  • 2.Pingdingshan University, Pingdingshan, Henan 467000, China
  • 3.Pingdingshan Bureau of Agriculture and Rural Affairs, Pingdingshan, Henan 467001, China
Published: 2025-10-15 doi: 10.7606/j.issn.1009-1041.2025.10.06
Outline
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Wheat germplasms are the important foundation for wheat breeding. In this study, the genetic diversity was analyzed for 40 wheat germplasms from Pingdingshan region using SSR molecular markers. Results showed that the percentage of polymorphic bands for SSR primers ranges from 35% to 100%, with an average of 74.5%. A total of 164 polymorphic bands were obtained by 29 pairs of SSR primers, with an average of 5.60 bands for a single pair of SSR primers. The effective number of alleles, Nei's gene diversity index, and Shannon's information index of SSR primers ranged from 1.17 to 1.66, from 0.11 to 0.38, and from 0.17 to 0.55, respectively, with the average values of 1.43, 0.26, and 0.39, respectively. The genetic similarity coefficient between different wheat germplasms is distributed between 0.55 and 0.98, with an average of 0.77. Based on the genetic similarity coefficient, the 40 wheat germplasms can be divided into five groups. Of them, group I contained three wheat germplasms; group Ⅱ contained eleven wheat germplasms; group Ⅲ contained five wheat germplasms; and group IV contained three wheat germplasms. Group Ⅴ contained the largest number (eighteen) of wheat germplasms, accounting for 45.0% of the total wheat germplasms. The result of principal component analysis was basically consistent with the results of cluster analysis. In addition, DNA fingerprints of the 40 wheat germplasms were constructed with SSR primers.

Wheat  /  SSR molecular marker  /  DNA fingerprint  /  Genetic diversity
王二伟, 马爱锄, 王健胜, 杨好好, 王军峰, 黄雅敏, 耿若飞, 蒋钦群. 平顶山地区小麦种质资源DNA指纹图谱构建及遗传多样性分析. 麦类作物学报, 2025 , 45 (10) : 1342 -1350 . DOI: 10.7606/j.issn.1009-1041.2025.10.06
Erwei WANG, Aichu MA, Jiansheng WANG, Haohao YANG, Junfeng WANG, Yamin HUANG, Ruofei GENG, Qinqun JIANG. DNA Fingerprint Construction and Genetic Diversity Analysis of Wheat Germplasms in Pingdingshan Region[J]. Journal of Triticeae Crops, 2025 , 45 (10) : 1342 -1350 . DOI: 10.7606/j.issn.1009-1041.2025.10.06
Year 2025 volume 45 Issue 10
112
25
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Article Info
doi: 10.7606/j.issn.1009-1041.2025.10.06
  • Receive Date:2024-07-28
  • Online Date:2026-09-18
  • Published:2025-10-15
Article Data
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History
  • Received:2024-07-28
  • Revised:2024-12-02
Funding
Affiliations
    1.Pingdingshan Academy of Agricultural Science, Pingdingshan, Henan 467001, China
    2.Pingdingshan University, Pingdingshan, Henan 467000, China
    3.Pingdingshan Bureau of Agriculture and Rural Affairs, Pingdingshan, Henan 467001, China
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表12种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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