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Outline
In order to screen for high callus induction rate wheat and improve the efficiency of wheat anther culture in vitro, 22 wheat materials were selected for callus induction rate using W14mf medium. Based on W14mf medium, different concentrations of 2,4-D (1.0, 1.5, 2.0, 2.5, and 3.0 mg·L-1), 6-BA (0, 1.0, 2.0, 3.0, and 4.0 mg·L-1), ABA (0, 0.2, 0.3, and 0.4 mg·L-1), and arabinogalactan protein (0, 10.0, 50.0, 200.0, and 400.0 mg·L-1) were added to analyze the effects of hormones and carbon sources on callus induction rate of different wheat genotypes (Jimai 262, Jimai 38, and Jinuo 116). The results showed that five materials with callus induction rates over 50% (Jimai 221001, Yannong 1212, Shimai 366, Jimai 35, and Yannong 999) were screened using W14mf medium, with callus induction rates of 132%, 87%, 84%, 81%, and 76%, respectively. When only adjusting the concentration of 2,4-D, Jimai 262 had the highest callus induction rate in the tested materials at 2.0 mg·L-1, while Jimai 38 and Jinuo 116 had the highest callus induction rates at 1.0 mg·L-1 and 1.5 mg·L-1, respectively. When only adjusting the concentration of 6-BA, Jimai 262 had the highest callus induction rate at 1.0 mg·L-1, while Jimai 38 and Jinuo 116 had the highest callus induction rates at 0 mg·L-1 and 2.0 mg·L-1, respectively. When only adjusting the concentration of ABA addition, the highest callus induction rate was observed for Jimai 4072 at 0.2 mg·L-1, and for Jinan 17 at 0 mg·L-1 addition. The results indicate that the addition levels of 2,4-D, 6-BA, and ABA are greatly influenced by genotype, and the concentrations of addition vary among different varieties. When only adjusting the concentration of arabinogalacto protein (AGP) addition, all tested materials showed the highest callus induction rate at 400.0 mg·L-1, and compared to 0 mg·L-1 addition, the callus induction rate of Jimai 38 and Jimai 4075 were increased by 1.5 and 8.0 times under 400.0 mg·L-1 AGP. The callus induction rate of Jimai 22 was 4.5% under 400.0 mg·L-1 AGP, achieving a breakthrough of 0 callus induction rate in the original culture medium W14mf. In addition, the callus induction rates of the four wheat materials tested were also improved. This result indicates that AGP can break the genotype limitation and effectively improve the callus induction rate of wheat anther culture.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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