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In order to further analyze the regulation mechanism of avenin-like proteins in flour quality of wheat, the TaALP-7D gene was successfully cloned from Zhengmai 158 (strong dough strength). Relative expression patterns of different tissues showed that the TaALP-7D gene was highly expressed at different developmental stages of the kernels. It was suggested that TaALP-7D gene may be involved in the regulation of genes related to dough strength during wheat seed development. Six possible candidate interactive proteins of TaALP-7D were screened from Zhengmai 158 cDNA library by the yeast two-hybrid system: cysteine hydrolase 1 (XM_044496235.1), F-box protein SKP2A-like (XM_044518121.1), non specific lipid transfer protein LTP1-like (XM_044488525.1), and 1,3-β-glucosidase (XM_044596055.1), and also the ethylene response transcription factor TaERF1-like (XM_044549609.1) and jasmonic acid-induced oxidase JOX2-like (XM_044519612.1) related to plant hormone response. The rotation validation assays of the five representative candidate proteins (cysteine hydrolase 1, F-box protein SKP2A like, non-specific lipid transfer protein LTP1-like, 1,3-β-glucosidase, and ethylene response transcription factor TaERF1-like) indicated that all of them interacted with TaALP-7D. Therefore, it is speculated that TaALP-7D probably interacts with these candidate proteins and be mainly involved in the degradation of storage proteins (such as gliadins), synthesis of starch in wheat kernels, as well as other processes of plant hormone response
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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