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As a unique and important germplasm resource in China, the Taigu male-sterile wheat has made significant contributions to wheat breeding. To further explore its potential application, this study aimed to establish an effective seed-stage identification system for the Taigu genic male sterility trait (Ms2) using molecular biology techniques, employing DsRed (encoding red fluorescent protein) and RUBY (a pigment biosynthesis-related gene) as reporter systems. Additionally, the single-copy semi-dwarf gene Rht-D1b was introduced to develop semi-dwarf, visually identifiable male sterile wheat. Through characterization of transgenic wheat plants and their progeny, the red fluorescent protein was efficiently expressed and stably inherited in wheat embryos. Although RUBY expression was undetected, effective identification of male sterility could still be achieved through red fluorescence. Sterile seeds with red fluorescence and fertile seeds without fluorescence exhibited a 1∶1 segregation ratio. T1 plants derived from red fluorescent seeds were all semi-dwarf and sterile, while those from non-fluorescent seeds were non-dwarf and fertile, indicating tight linkage among DsRed, Ms2, and Rht-D1b. The constructed reporter system not only enables fertility sorting at the seed stage via fluorescence but also addresses the excessive dwarfing issue in current dwarf-sterile wheat lines by incorporating the semi-dwarfing gene. This approach offers a novel tool for hybrid wheat production and recurrent selection breeding.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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