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Cloning and Function Analysis of E3 Ubiquitin Ligase Gene TaPUB23 in Wheat
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Hai LI, Yunshuang LU, Yifeng SHI, Yuxiang LI, Xiaoping HU
Journal of Triticeae Crops | 2026, 46(2) : 154 - 163
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Journal of Triticeae Crops | 2026, 46(2): 154-163
• Genetics & Breeding •
Cloning and Function Analysis of E3 Ubiquitin Ligase Gene TaPUB23 in Wheat
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Hai LI, Yunshuang LU, Yifeng SHI, Yuxiang LI, Xiaoping HU
Affiliations
  • Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loss Plateau, Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China
Published: 2026-02-15 doi: 10.7606/j.issn.1009-1041.2026.02.02
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To investigate the molecular mechanism of high-temperature all-stage stripe rust resistance in Xiaoyan 6, an E3 ubiquitin ligase gene, TaPUB23, was identified based on the previous transcriptome sequencing results. This gene, which was significantly upregulated under both stripe rust infection and high-temperature conditions, has a full-length sequence of 1,272 bp, encoding 423 amino acids, and is localized in the cell membrane, cytoplasm, and nucleus. Virus-induced transient overexpression of TaPUB23 significantly reduced the stripe rust resistance of Xiaoyan 6 plant. Based on the yeast two hybrid (Y2H) system, we identified the interaction target TaDJA7 of TaPUB23 and demonstrated that TaPUB23 ubiquitinated TaDJA7. The interaction between TaPUB23 and TaDJA7 was further validated through luciferase complementation assays (LCA), bimolecular fluorescence complementation (BiFC), and Pull-down assays. These findings reveal that the E3 ubiquitin ligase TaPUB23 regulates high-temperature all-stage stripe rust resistance in wheat through ubiquitination of TaDJA7.

Wheat stripe rust  /  E3 ubiquitin ligase  /  TaPUB23  /  TaDJA7
Hai LI, Yunshuang LU, Yifeng SHI, Yuxiang LI, Xiaoping HU. Cloning and Function Analysis of E3 Ubiquitin Ligase Gene TaPUB23 in Wheat[J]. Journal of Triticeae Crops, 2026 , 46 (2) : 154 -163 . DOI: 10.7606/j.issn.1009-1041.2026.02.02
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Year 2026 volume 46 Issue 2
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doi: 10.7606/j.issn.1009-1041.2026.02.02
  • Receive Date：2025-03-28
  • Online Date：2026-09-11
  • Published：2026-02-15
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  • Received：2025-03-28
  • Revised：2025-04-29
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    Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loss Plateau, Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China
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https://castjournals.cast.org.cn/joweb/mlzwxb/EN/10.7606/j.issn.1009-1041.2026.02.02
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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