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Cytokinins (CKs) play a pivotal regulatory role in plant growth, development and stress responses, with isopentenyltransferases (IPTs) serving as critical rate-limiting enzymes in CKs biosynthesis. To investigate the role of wheat IPT genes in plant responses to low-temperature stress, this study cloned the TaIPT9A gene (TraesCS7A02G560300) from the tiller nodes of the cold-resistant winter wheat cultivar Dongnong Dongmai 1(Dn1). Bioinformatics analysis, low-temperature-induced promoter activity assays, and functional characterization were conducted. The TaIPT9A gene was overexpressed in the cold-sensitive rice cultivar Longjing 11 via Agrobacterium-mediated transformation to preliminarily analyze its cold resistance function. The results showed that TaIPT9A has a coding sequence (CDS) of 1 362 bp, encoding 453 amino acids. Phylogenetic analysis revealed that wheat IPT proteins are most closely related to those of Aegilops tauschii. The TaIPT9A promoter contains cis-acting elements responsive to low temperature, light, ABA and MeJA, and its activity was induced by cold stress. Compared to wild-type rice, TaIPT9A-overexpressing plants exhibited greener leaves and better growth under low-temperature stress. Additionally, these plants showed significantly increased proline (Pro) content, catalase (CAT) activity, and upregulated expression of antioxidant enzyme-related genes (OsCATC, OsSOD1, OsSOD2 and OsAPX1) as well as cold resistance-related genes (OsCATC and OsDREB1A). These findings suggest that TaIPT9A enhances cold tolerance in rice seedlings.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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