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To investigate the effects of spermidine on the starch composition and gelatinization characteristics of wheat grains, using the wheat varieties Xinong 538 and Lankaoaizao 8 as experimental materials, four spermidine spray concentrations (0.25, 0.75 and 1 mmol·L-1) were set up at grain-filling stage. The indicators of starch composition, granule size distribution, and gelatinization characteristics of wheat grains were determined and analyzed. The results showed that compared with spraying clear water CK, spraying 0.25 and 0.75 mmol·L-1 spermidine significantly increased the 1 000-grain weight and yield of the two varieties. After the application of spermidine, the amylose content of Xinong 538 and Lankaoaizao 8 decreased and increased, respectively. The amylopectin ratio showed a decreasing trend and a downward increase followed by a decrease with the increase of the application concentration. Overall, spermidine spraying reduced the quantity and volume of A type starch granules in both varieties and increased the quantity and volume of B type starch granules. High concentration (1 mmol·L-1) of spermidine reduced, increased and decreased the peak viscosity, final viscosity, and breakdown of Xinong 538 and Lankaoaizao 8, respectively. 0.75 mmol·L-1 spermidine increased the peak viscosity, trough viscosity, breakdown, final viscosity, and setback of Lankaoaizao 8, while increased the breakdown, setback, peak time, and pasting temperature of Xinong 538. Overall, under the conditions of this experiment, the application of 0.75 mmol·L-1 arginine spray significantly increased the 1 000-grain weight and yield of wheat, and by influencing the amylose content, amylose/amylopectin ratio and type B starch particle composition of wheat, increased the peak viscosity and breakdown of wheat grain starch, thereby enhancing the gelatinization characteristics of wheat and ultimately improving the quality of wheat starch. It is a relatively suitable spraying concentration in wheat production.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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