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Cloning of TaDGAT and Analysis of Its Potential Function in Anther Development in Wheat
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Changjing CHEN1, 2, Shengkai YING2, Yingying XU2, Nuo ZHAI2, Zihan LIU2, Yongjie LIU2, Liping ZHANG1, 2, Xiangzheng LIAO2, Qingpeng SUN1, Jianfang BAI2
Journal of Triticeae Crops | 2026, 46(5) : 604 - 612
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Journal of Triticeae Crops | 2026, 46(5): 604-612
• Genetics & Breeding •
Cloning of TaDGAT and Analysis of Its Potential Function in Anther Development in Wheat
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Changjing CHEN1, 2, Shengkai YING2, Yingying XU2, Nuo ZHAI2, Zihan LIU2, Yongjie LIU2, Liping ZHANG1, 2, Xiangzheng LIAO2, Qingpeng SUN1, Jianfang BAI2
Affiliations
  • 1.College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China
  • 2.Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing, 100097, China
Published: 2026-05-15 doi: 10.7606/j.issn.1009-1041.2026.05.05
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To explore the potential function of diacylglycerol acyltransferase (DGAT) in the development of anther, the wheat photothermo-sensitive male sterile line BS366 was used as the material. Based on previous transcriptome sequencing results, TaDGAT (TraesCS1A02G125300) was screened and cloned from BS366. Bioinformatics analysis was employed to predict the structural and functional characteristics of the TaDGAT gene and its encoded protein. The results indicated that the gene has an open reading frame (ORF) of 1 542 bp, encoding 513 amino acids. Based on protein structure, the protein was predicted to be an O-acyltransferase. Multiple sequence alignment and phylogenetic tree analysis revealed that TaDGAT is most closely related to those in wheat and barley. Additionally, miRNAs, such as miR398 and miR169, were found to potentially interact with TaDGAT, participating in lipid metabolism processes and thereby regulating anther development. Protein-protein interaction predictions identified key interactions with O-acyltransferase and other critical proteins in lipid metabolism pathways. Subcellular localization prediction indicated that TaDGAT is located in the cell membrane and Golgi apparatus, where it participates in the formation of biological membranes. Expression pattern analysis showed that the expression of TaDGAT in different tissues, the level in anthers is low, while it is highly expressed under sterile conditions. This also suggested that the gene is closely related to pollen formation. This study provides certain references for elucidating the function of the DGAT gene in wheat.

Wheat  /  Photothermal-sensitive male sterility  /  DGAT  /  Expression analysis  /  Fluorescence quantitative PCR
Changjing CHEN, Shengkai YING, Yingying XU, Nuo ZHAI, Zihan LIU, Yongjie LIU, Liping ZHANG, Xiangzheng LIAO, Qingpeng SUN, Jianfang BAI. Cloning of TaDGAT and Analysis of Its Potential Function in Anther Development in Wheat[J]. Journal of Triticeae Crops, 2026 , 46 (5) : 604 -612 . DOI: 10.7606/j.issn.1009-1041.2026.05.05
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Year 2026 volume 46 Issue 5
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Article Info
doi: 10.7606/j.issn.1009-1041.2026.05.05
  • Receive Date：2025-10-31
  • Online Date：2026-09-11
  • Published：2026-05-15
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History
  • Received：2025-10-31
  • Revised：2026-01-20
Funding
Affiliations
    1.College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China
    2.Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing, 100097, China
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https://castjournals.cast.org.cn/joweb/mlzwxb/EN/10.7606/j.issn.1009-1041.2026.05.05
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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