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To explore the structural differences of 1RS arms in wheat-rye 1BL·1RS translocation cultivars (lines), the 1BL·1RS translocation cultivar Nanmai 618 was used as parent to hybridize with 13 1BL·1RS translocation cultivars (lines), including Aikang 58, Chuannong 17 and Lankao Aizao 8, as well as the 7DL·1RS translocation cultivar Lumai 8. A total of 14 types of F1 plants were obtained. High-GC content oligonucleotide probes (Oligo-119.2-2, 200G, 250G) and high-AT content oligonucleotide probes (Oligo-119.2-3, 200T, 250T) were designed based on the tandem repeats pSc119.2, pSc200, and pSc250. These probes were used in non-denaturing fluorescence in situ hybridization (ND-FISH) analysis for identifying the metaphase chromosomes of root tips from these F1 plants. The results showed that, except for Oligo-119.2-3 which produced no signal, the other five probes generated signals with varying intensities on 1RS chromosome arms from different sources. The signal intensities of probes 200G and 200T on 1RS exhibited consistent variation trends, while the variations reflected by 250G and 250T were inconsistent. For example, 250T showed distinct signal intensities on 1RS from different sources, whereas 250G displayed similar signal intensities on the corresponding 1RS arms. These findings indicated that there were differences in the GC and AT base contents of the pSc250 tandem repeated family in 1RS among different cultivars. According to the variation in signal intensity of the probes on 1RS, the 1RS arms in these 15 wheat cultivars (lines) could be classified into four structural types. The results of this study revealed the structural variations of 1RS, which enriches the understanding of 1BL·1RS translocation chromosomes in wheat cultivars (lines) in China.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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