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Gene Cloning and Bioinformatics Analysis of Wheat TaPBF3-5D
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Yunhao DENG1, 2, Luhua LI1, 2, Ling XU1, 2, Zhongni WANG3, Dingli HONG1, 2, Chengjiang TAI1, Ruhong XU1, 2
Journal of Triticeae Crops | 2025, 45(2) : 141 - 148
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Journal of Triticeae Crops | 2025, 45(2): 141-148
• Genetics & Breeding •
Gene Cloning and Bioinformatics Analysis of Wheat TaPBF3-5D
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Yunhao DENG1, 2, Luhua LI1, 2, Ling XU1, 2, Zhongni WANG3, Dingli HONG1, 2, Chengjiang TAI1, Ruhong XU1, 2
Affiliations
  • 1.College of Agriculture, Guizhou University, Guiyang, Guizhou 550025, China
  • 2.Guizhou Branch of National Wheat Improvement Center, Guiyang, Guizhou 550025, China
  • 3.Rice Research Institute of Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550025, China
Published: 2025-02-15 doi: 10.7606/j.issn.1009-1041.2025.02.01
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PBF factor is one of the protein factors regulating the expression of wheat endosperm specific gene, and plays an important role in regulating the expression of wheat seed storage protein genes. In this study, the CDS sequence of TaPBF3-5D was obtained from wheat germplasm ZY96-3 by RT-PCR, and its bioinformatics and gene expression were analyzed. The results showed that the CDS sequence of TaPBF3-5D had a total of 909 bp, encoding 302 amino acids. The encoded protein is a non-transmembrane unstable hydrophilic protein without signal peptide, and it contains 36 phosphoric acid sites. It is predicted to be located in the nucleus. Phylogenetic tree and multiple sequence alignment showed that TaPBF3-5D was more closely related to the PBF from common wheat. Quantitative fluorescence analysis showed that TaPBF3-5D gene was expressed in roots, stems, leaves, and seeds, with the highest expression in seeds, followed by leaves and stems, and the lowest expression in roots. It indicated TaPBF3-5D gene regulating the grain development of wheat through participating in the regulation of wheat grain storage protein gene expression.

ZY96-3  /  TaPBF3-5D  /  Bioinformatics  /  Gene cloning
Yunhao DENG, Luhua LI, Ling XU, Zhongni WANG, Dingli HONG, Chengjiang TAI, Ruhong XU. Gene Cloning and Bioinformatics Analysis of Wheat TaPBF3-5D[J]. Journal of Triticeae Crops, 2025 , 45 (2) : 141 -148 . DOI: 10.7606/j.issn.1009-1041.2025.02.01
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Year 2025 volume 45 Issue 2
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Article Info
doi: 10.7606/j.issn.1009-1041.2025.02.01
  • Receive Date：2024-04-01
  • Online Date：2026-09-11
  • Published：2025-02-15
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History
  • Received：2024-04-01
  • Revised：2024-08-14
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Affiliations
    1.College of Agriculture, Guizhou University, Guiyang, Guizhou 550025, China
    2.Guizhou Branch of National Wheat Improvement Center, Guiyang, Guizhou 550025, China
    3.Rice Research Institute of Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550025, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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