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In order to improve the construction of wheat DH lines, the F1 generation of 11 wheat hybrid combinations was used as materials to compare and analyze the induction efficiency of wheat anthers under two media (NPB-99 and CHB) and the chromosome doubling efficiency of haploids under three concentrations of colchicine treatments (4, 6 and 8 g·L-1). The high-molecular-weight glutenin subunits (HMW-GS) of the obtained DH lines were identified by SDS-PAGE. The results showed that the anther induction efficiency of CHB medium was better than that of NPB-99 medium. The concentration of 6 g·L-1 colchicine was more efficient in inducing haploid doubling. A total of 16 lines carrying prior HMW-GS combinations (1/7+8/5+10 or 1/7+9/5+10) were identified from the 33 DH lines, with good baking quality, which could provide genetic resources for subsequent wheat quality breeding research.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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