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NAC transcription factors play an important role in plant response to stress. In order to further understand the biological functions of salt-tolerant NAC transcription factors in wheat, the transcriptome of salt-tolerant material Cangmai 6005 and salt-sensitive material Kenong 9204 were sequenced. NAC transcription factors related to salt tolerance were screened and bioinformatics analyzed. RT-PCR was used to identify the key candidate genes by reverse transcription of cDNA and RNA extracted from the roots under salt stress. The results showed that among the 28 NAC transcription factors, 10 had alkaline isoelectric points and the others had acidic isoelectric points, and all of them were hydrophilic proteins. The protein secondary structure is mainly irregular curl and α-helix. In the collinearity analysis, there were many pairs of collinearity in wheat and rice, indicating that the close relationship between wheat and rice. The promoter region of NAC transcription factor contains many cis-regulatory elements related to hormone response and stress induction, among which TaNAC24 has the most (51) cis-regulatory element binding sites. The results of RT-qPCR showed that the expression levels of four genes were significantly up-regulated and five genes were significantly down-regulated after salt stress treatment, which was consistent with the results of transcriptome sequencing. These genes may mediate the response of wheat to salt stress.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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