In order to effectively reduce the impact of Fusarium head blight (FHB) on wheat production in southwestern China and explore resistance germplasm resources and genes against FHB, the study was conducted from 2022 to 2024 on agronomic traits such as thousand grain weight, plant height and grain number per ear of 80 wheat varieties (lines) grown in Guizhou. Single flower drip inoculation method was used to identify the phenotype resistance of FHB in the field, combined with 11 pairs of molecular markers closely linked to Fhb1, Fhb2, Fhb4, Fhb5, Fhb7 and QFhs.crc-2DL for detection. The molecular detection results showed that 59 varieties (lines) carried disease resistance genes, among which the frequencies of Fhb1, Fhb2, Fhb4, Fhb5, Fhb7 and QFhs.crc-2DL were 37.50%, 28.75%, 22.50%, 40.00%, 2.50% and 23.75%, respectively. A total of 37 varieties (lines) simultaneously carried 2 to 4 disease resistance genes, with the combinations of Fhb1+Fhb2+Fhb5 and Fhb1+Fhb5 exhibiting detection frequencies of 8.75% and 6.25%, respectively. After two years of resistance identification, a total of 11 resistant varieties (lines) were screened. Comprehensive analysis revealed that three varieties (lines), namely Qian 0938-3, Qian 15168 and Qian 13 Xia 163F8-5, performed good disease resistance and excellent agronomic traits, which can be used as germplasm resources for genetic improvement of wheat resistance to FHB.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |