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In order to screen salt-tolerant germplasm of oat, 200 oat germplasm resources were subjected to complexed salt stress (NaCl∶Na2SO4∶NaHCO3∶Na2CO3 = 1∶9∶9∶1). Six germination indices such as seed vigor index, shoot length and root length were measured. The salt-tolerant ability of oat was comprehensively evaluated by principal component analysis (PCA) and cluster analysis. The results showed that the combined saline-alkali stress significantly inhibited the germination and early growth of oat germplasm. The germination rate, germination energy, root length, shoot length, and seed vigor index all decreased to varying degrees, among which the vigor index decreased the most, while the salt damage rate increased significantly. Two principal components were extracted by PCA, and the cumulative variance contribution rate was 81.57%. Principal component 1 exhibited a higher loading on seed vigor index, shoot length, root length, and germination rate, reflecting traits related to the germination potential of oats. Principal component 2 was mainly associated with salt injury rate and root length, reflecting the tolerance performance of seeds under salt-alkali stress. Based on the comprehensive evaluation value (D value) in clustering analysis, the germplasms were classified into five groups: highly salt-alkali tolerant group (D≥0.850, 2 germplasms), salt-alkali tolerant group (0.850>D≥0.720, 2 germplasms), moderately salt-alkali tolerant group (0.720>D≥0.460, 15 germplasms), salt-alkali sensitive group (0.460>D≥0.305, 58 germplasms), and extremely salt-alkali sensitive group (D<0.305, 123 germplasms). Two oat germplasm lines with high tolerance (163 and 21) and two oat germplasm lines with tolerance (B106 and B60) to salt-alkali stress were screened.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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