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To investigate the effects of a self-developed compound agricultural probiotic inoculant on wheat growth, yield and grain quality, a field experiment was conducted using a new wheat line Dunmai 99-1 during the 2023—2024 and 2024—2025 growing seasons. Under a spraying volume of 250 L per plot (area 85.8 m2) each time, five compound inoculant application levels of 0, 25, 5, 25 ahd 50 L were set, and represented by CK, T1, T2, T3 and T4, respectively. The inoculant was applied five times through drip irrigation from the seedling stage to the grain-filling stage. Agronomic traits, aboveground fresh weight, antioxidant enzyme activities and malondialdehyde content were determined at different growth stages, and yield components, grain yield, and grain quality traits were measured at maturity. The results showed that the compound inoculant significantly promoted wheat growth at the tillering, jointing and booting stages, and exhibited significant yield increasing effects. Compared with the control, the yield increase ranked as T3>T4>T2>T1. Among them, T3 showed the greatest yield advantage, with grain yields of 11 258.56 and 12 046.48 kg·hm-2 in the two growing seasons, increased by 25.59% and 23.63%, respectively, compared to the control. In terms of grain quality, except that the starch content under T1 was slightly lower than that of CK in the 2024—2025 season, the starch content, crude protein content, wet gluten content, and test weight under the other treatments were generally higher than those of CK. Most grain quality traits showed an increasing trend with increasing inoculant application within a certain range, and T4 showed the best overall performance. In conclusion, the self-developed compound agricultural probiotic inoculant showed good potential for promoting wheat growth, increasing yield, and improving grain quality.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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