PDF
Full
Outline
To elucidate the functions of the wheat mitogen-activated protein kinase genes TaMAPK1-6A, TaMAPK1-6B, and TaMAPK1-6D in seedling drought tolerance and the additive effects among these homeologous genes, this study utilized the spring wheat cultivar Fielder and its CRISPR/Cas9 mediated single-gene mutants-mapk1-aa, mapk1-bb, and mapk1-dd-generated in the Fielder genetic background. Through hybridization, self-pollination, and genotyping of segregating progeny, double-gene knockout mutants and triple-gene knockout homozygous mutants (hereafter referred to as double and triple mutants) were successfully developed. The drought tolerance at the seedling stage was systematically evaluated for these single, double, and triple mutants, along with the wild-type Fielder control. The results demonstrated that loss-of-function mutations in single TaMAPK1 genes significantly reduced drought tolerance in wheat seedlings, manifested as decreased leaf water retention, reduced accumulation of osmotic adjustment substances, and enhanced membrane lipid peroxidation. Further investigation revealed that drought tolerance was further impaired in double and triple mutants, with the deterioration of physiological and biochemical indicators intensifying as the number of mutated gene copies increased. In summary, the TaMAPK1 gene positively regulates drought tolerance at the wheat seedling stage, and its homeologous genes exhibit cumulative regulatory effects.
PDF
39
14
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
