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Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), severely influences wheat yield and grain quality. Mining novel powdery mildew resistance (Pm) genes and developing functional markers is of great significance for molecular breeding of disease-resistant wheat. In this study, an F2 segregating population was first constructed using Hongyoumai as the resistant parent and Ningmaizi 119 as the susceptible parent. By integrating phenotypic data from resistance identification, diagnostic molecular marker development, and recombinant analysis, the wheat powdery mildew resistance gene PmHYM was finely mapped. Subsequently, sequence characteristics of the candidate genes were analyzed through sequence alignment, flow sorting and chromosome sequencing, expression pattern analysis, and copy number variation detection. Finally, KASP markers were developed based on functional SNP, and the breeding potential of the gene was evaluated via backcross breeding. The results showed that PmHYM was fine-mapped to a 0.5 cM genetic interval between markers Xmp1410 and Xmp1396 on chromosome 7BL, corresponding to a 310 kb physical interval in the Chinese Spring reference genome. Two coiled-coil nucleotide-binding leucine-rich repeat (CNL)-type proteins were predicted within this region. The sequences of these two genes in Hongyoumai were identical to those in Fuzhuang 30. The difference in the resistance spectrum to Bgt isolate B16, between Hongyoumai and Fuzhuang 30 was independent of gene sequence variations or copy number variations. A high-throughput diagnostic KASP marker Xmp1394 for PmHYM was successfully developed, enabling rapid tracking of PmHYM. Backcross-derived lines carrying PmHYM exhibited no significant differences in agronomic traits compared with the recurrent parent, suggesting as high potential for breeding application.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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