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Using resistance genes is the most effective measure to control wheat leaf rust. The wheat leaf rust resistance gene Lr15 showed resistance to most Puccinia triticina (Pt) races in China. Further confirmation of the genetic characteristics and position of Lr15 will lay a foundation for marker-assisted breeding and gene cloning. Firstly 13 Pt races were used to test the seedling resistance line RL6052 with Lr15. Furthermore, 257 F2 plants derived from a cross between RL6052 and the susceptible cultivar Thatcher were inoculated with race PHST for resistance evaluation, and bulked segregant analysis using a 16K SNP array was performed for preliminary mapping of Lr15. Finally, SSR and STS markers were developed within the target region to construct a genetic linkage map for Lr15. The results showed that RL6052 was highly resistant to 12 races. Genetic analysis revealed that the ratio between resistant to susceptible was 192∶65 in the F2 population, fitting a 3∶1 ratio (=0.012), indicating monogenic dominant inheritance. Lr15 was mapped on a 60.16-87.26 Mb physical interval on chromosome 2DS in the Chinese Spring reference genome (version 1.0) using a 16K SNP array. More markers were developed in this interval and Lr15 was mapped between markers ZBSF63.7 and STS64.4, with a physical interval of 61.27-62.02 Mb, and genetic distances of 0.17 and 0.58 cM, respectively. Two markers ZBSF2D63.9 and ZBSF2D63.92 were co-segregated with Lr15. In this study, the effective resistance gene Lr15 was precisely mapped on chromosome 2DS and closely linked markers were also developed, which laid a foundation for further cloning of this gene, and the closely linked markers can be used for marker-assisted selection for breeding resistant cultivars.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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