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In order to explore the feasibility of applying the gluten-starch interaction to breed super-gluten wheat variety, 20 F7recombinant inbred lines (RILs) derived from a cross between Jimai 44 and Jimai 229 and their parents were used as materials, and the farinograph properties of dough were measured. Three RILs with the longer stability time (Group H) and 3 RILs with the shorter stability time (Group L) were selected for further invistigating the gluten protein quantity and quality, starch size distribution and gluten-starch interaction. The results showed that the Group H exhibited higher dough development time and stability time than those of their parents, which can be attributed to their tighter gluten network, higher B-type starch granule proportion and stronger gluten-starch interaction. Correlation analysis showed that dough development time and stability time had significantly positive correlation with gluten index, protein junctions, protein length, B-type starch granule proportion, the ratio of B-type starch granules to A-type starch granules (B/A), B/lacunarity, and B/A/lacunarity, yet significantly negative correlation with lacunarity, A-type starch proportion and A/lacunarity. In summary, compared with Jimai 44 and Jimai 229, the gluten network structure, B-type starch granule proportion, and gluten-starch interaction of Group H were significantly improved. Parameters related to gluten-starch interaction determined using micro-scale flour samples can be used to assess the dough quality at an early generation in wheat breeding.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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