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Hulless barley is a crop of significant economic and ecological value in the Qinghai-Tibet Plateau. Establishing a core germplasm resource bank based on its nutritional quality is crucial for promoting genetic improvement and the efficient utilization of hulless barley germplasm resources. In this study, a total of 286 hulless barley germplasm resources were selected, and a core germplasm subset was constructed based on 15 quality traits, including total flavonoid content. A multidimensional combination strategy was employed, incorporating two genetic distances, three sampling methods, seven sampling ratios, and eight clustering methods. By comparing the mean difference percentage, variance difference percentage, range conformity rate, and coefficient of variation change rate for various subsets, the most optimal sampling strategy for constructing the hulless barley core germplasm was identified. This strategy involved Euclidean distance, a multiple clustering deviation sampling method, a 10% sampling ratio, and the shortest distance method. This resulted in the establishment of a core germplasm bank consisting of 28 hulless barley germplasms based on nutritional quality traits. To validate the representativeness of the constructed core germplasm, key statistical parameters, including the mean, variance, range, coefficient of variation, diversity index, principal component analysis, and clustering analysis, were analyzed for both the original germplasm and the selected core germplasm across the 15 nutritional quality traits. The results showed no significant difference in the mean values of the 15 nutritional quality traits between the core and original germplasm. Moreover, the variance of each trait in the core germplasm was not lower than that of the original germplasm. The average diversity index conformity rate for the core germplasm reached 93.12%. Principal component analysis revealed that the cumulative contribution rate of the core germplasm was 82.04%, compared to 64.34% for the original germplasm. Cluster analysis further classified the 28 core germplasms into five distinct groups. The findings indicate that the core germplasm constructed in this study exhibits significant heterogeneity. It effectively retains most of the genetic information of the original germplasm while also representing the original germplasm adequately. This core germplasm can be utilized for further research on the nutritional quality of hulless barley and will contribute to the efficient use of germplasm resources.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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