Thousand-kernel weight is one of the key yield components in modern wheat breeding where significant progress has been made through genetic improvement. It is crucial to conduct in-depth research on the important genes controlling wheat kernel weight and their functions. In this study, molecular markers for five genes related to wheat kernel weight (TaSus2-2B, TaGW8-B1, TaCwi-A1, TaGS-D1, and TaGW2-6A) were used to test 263 new wheat varieties (lines) collected from the 2023-2024 national trials in the Huanghuai wheat region. Among these varieties (lines), all kernel weight-related genes existed in combinations. There were nine types of combinations for the five prior haplotypes, specifically: TaGW8-B1a+TaGW2-6A-A, TaSus2-2Ba+TaGW8-B1a+TaGW2-6A-A, TaGW8-B1a+TaGS-D1a+TaCwi-A1a, TaGW8-B1a+TaGS-D1a+TaGW2-6A-A, TaGW8-B1a+TaCwi-A1a+TaGW2-6A-A, TaSus2-2Ba+TaGW8-B1a+TaGS-D1a+TaGW2-6A-A, TaGW8-B1a+TaGS-D1a+TaCwi-A1a+TaGW2-6A-A, TaSus2-2Ba+TaGW8-B1a+TaCwi-A1a+TaGW2-6A-A, and TaSus2-2Ba+TaGW8-B1a+TaGS-D1a+TaCwi-A1a+TaGW2-6A-A. Among these combinations, the TaSus2-2Ba+TaGW8-B1a+TaGW2-6A-A combination had the lowest frequency at 1.90%, while the TaGW8-B1a+TaGS-D1a+TaCwi-A1a+TaGW2-6A-A combination had the highest frequency at 31.56%. Further evaluation and analysis of kernel traits in 2023 in Zhengzhou and Zhumadian and in 2024 in Xinxiang revealed that among these nine gene combination types, the TaGW8-B1a+TaGW2-6A-A combination exhibited a relatively weak effect on increasing wheat kernel weight, whereas the combination type with five pyramided superior kernel weight-related alleles(TaSus2-2Ba+TaGW8-B1a+TaGS-D1a+TaCwi-A1a+TaGW2-6A-A) showed a relatively strong effect on improving wheat kernel weight. The results of this study indicated that increasing the number of superior kernel weight-related genes(haplotypes) could effectively enhance the kernel weight of wheat varieties in the Huanghuai wheat region.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |