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To elucidate the promoter of the barley LRR-type receptor-like protein kinase gene HvLRR-RLK510 and its function, specific PCR primers were used to amplify the promoter of the HvLRR-RLK510 gene from Morex and Baodamai 8. The pEASY®-510 recombinant plasmid was constructed, and the promoter sequence of 2 368 bp was sequenced based on the positive colonies of the Escherichia coli Trans1-T1 strain. The prediction results from the online software PlantCARE indicated that the promoter contains response elements to non-biotic factors such as methyl jasmonate, abscisic acid, and low temperature. GUS staining results in roots, stems, leaves, and flowers of transgenic Arabidopsis thaliana and transiently expressed Nicotiana benthamiana plants demonstrated that the cloned promoter and its truncated fragments (-1 458, -868, and -460 bp) can equivalently drive the expression of the GUS gene in anthers. Quantitative real-time PCR (qRT-PCR) results showed that methyl jasmonate (MeJA), abscisic acid (ABA), low temperature, polyethylene glycol (PEG-2000), and light can effectively alter the expression characteristics of the HvLRR-RLK510 gene in barley leaves and roots. The promoter of the HvLRR-RLK510 gene cloned in this study is an inducible promoter containing response elements to jasmonic acid, abscisic acid, low temperature, drought, and light. Its -460 bp truncated fragment can replace the full-length promoter. The promoter and its truncated fragments can be used to elucidate the transcriptional regulatory mechanisms of the HvLRR-RLK510 gene.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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