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Fusarium head blight is a worldwide disease caused by Fusarium graminea, which seriously affects the yield and quality of wheat. The toxin of deoxynivetol (DON) produced by the grain infected by the pathogen poses a serious threat to human and animal health. In wheat, the toxicity of DON toxin is mainly caused by oxidative stress, and the main function of vitamin E in plants is antioxidant. In order to verify the effect of HGGT gene on reducing toxin accumulation in wheat grains, we developed the specific markers for HGGT gene and screened 23 varieties carrying HGGT gene from 133 national dominant varieties and some local varieties and lines. Combined with the data of two-year average diseased spikelet rate and grain DON toxin content, the correlation analysis showed that: the average diseased spikelet rate with HGGT gene was 20.47%, which was 22.67 percentage points lower than that without HGGT gene (P<0.01), indicating that HGGT gene also had a certain effect on the spread resistance of wheat scab. Under the conditions of no inoculation, the average grain DON toxin content of cultivars carrying HGGT gene was 0.12 mg·kg-1, which was 75.70% lower than that of cultivars without HGGT gene (P<0.05). Under inoculation conditions, the average grain DON toxin content of cultivars carrying HGGT gene was 5.51 mg·kg-1, which was 52.97% lower than that of cultivars without HGGT gene (P<0.01). The difference of DON toxin content in grains after inoculation and before inoculation was correlated with genotype data, and it was found that there was a significant difference in the changes of toxin content in grains between varieties carrying HGGT gene and those without HGGT gene (P<0.01), and the DON toxin accumulation with this gene was reduced by 51.93%. It was verified that HGGT gene had a significantly negative regulatory effect on the accumulation of DON toxin in wheat grains. Yangmai 18, Yangmai 33, Sumai 3, harboring HGGT, had the lower grain DON toxin content than the national standard GB2761-2011 (1 000 μg·kg-1) based on the phenotype of two years. This study could promote development of new wheat germplasm with low DON toxin.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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