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This study aimed to investigate the regulatory role of cell division cytokinin in wheat root morphological development and to identify key functional genes involved in root system architecture regulation. The seedlings of a wheat variety Jinmai 47 were treated with exogenous 0.05 μmol·L-1 6-benzylaminopurine (6-BA) to systematically analyze its effects on root phenotypic traits. The genotypic sensitivity of 110 wheat varieties (lines) to 6-BA was evaluated. Furthermore, the TaCKX1B gene was obtained through homology-based cloning, and its function was validated via heterologous overexpression in tobacco. The results showed that the treatment with 0.05 μmol·L-1 6-BA significantly inhibited primary root elongation while promoting root hair initiation and elongation. Considerable genetic variation in sensitivity to 6-BA was observed among wheat genotypes, with sensitivity indices predominantly ranging from 40% to 60%. Materials with low sensitivity at the seedling stage exhibited more developed root systems at the grain-filling stage. The TaCKX1B gene was located on chromosome 1B, with a coding sequence length of 1 569 bp. Heterologous overexpression of TaCKX1B in tobacco resulted in approximately a two-fold increase in root biomass in T1 transgenic plants compared to wild-type controls. In conclusion, the TaCKX1B gene plays a positive regulatory role in plant root development.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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