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To explore the effect of salicylic acid (SA) on male fertility of thermo-sensitive genic male sterile wheat line BNS366, BNS366 and its near-isogenic line Zhengmai 366 were used as materials, which were sown under conditions of normal sowing in autumn (from October 10 to October 13) and late sowing (on December 1), respectively. In the growth seasons of 2021-2022 and 2022-2023, indirect enzyme-linked immunosorbent assays (ELISA) was used to detect the endogenous SA content in young panicles and anthers from the differentiation stage of pistil and stamen primordium to anthesis. In the growth season of 2023-2024, the wheat was sprayed with 6 different concentrations (0, 0.1, 0.5, 1.0, 1.5 and 2.0 mmol·L-1 ) of SA solution. The SA treatments began from the connective stage that the length of the spike was 1.0 cm and stopped just before anthesis. I2-KI method was used to detect frequency of fertile pollen grains at anthesis stage. National and international methods were used to determine the self-seeding rate. The results showed that in the growth seasons of 2021-2022 and 2022-2023, compared with Zhengmai 366 sown on the same date, under the conditions of normal sowing in autumn (in the growth seasons of 2021-2022 and 2022-2023) and late sowing (in the growth season of 2021-2022), the content of SA in young panicles of BNS366 (completely or high level of male sterility) was lower from the differentiation stage of pistil and stamen primordium to the tetrad stage, and higher in anthers at the uninucleate stage with small-vacuole and the trinucleate stage, and for BNS366 (fertility) under the conditions of late sowing (in the growth season of 2022-2023), the SA content was higher in panicles at the connective stage and in anthers at the uninucleate stage with small-vacuole. In the growth season of 2023-2024, under the conditions of normal sowing in autumn and late sowing, under the treatment of 2.0 mmol·L-1 SA, the international self-seeding rate of Zhengmai 366 was significantly increased by 28.16 and 31.25 percentage points than CK (0 mmol·L-1), respectively, and under the conditions of normal sowing in autumn, the national and international self-seeding rate of BNS366 was significantly increased by 1.25 and 1.76 percentage points than CK, respectively. However under the conditions of late sowing, under the treatment of 0.5 mmol·L-1 SA, the frequency of fertile pollen grains of BNS366 was significantly decreased by 37.97 percentage points than CK. Under the two sowing conditions, there were no significant differences in the three indices above mentioned of Zhengmai 366 and BNS366 under others SA treatments compared with CK. It was indicated that the lack of SA from the differentiation stage of pistil and stamen primordium to the tetrad stage might promote the occurrence of male-sterility; furthermore the exogenous SA increased the self-seeding rate of Zhengmai 366 (fertility) and BNS366 (male sterility), and decreased the frequency of fertile pollen grains of BNS366 (fertility).
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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