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Highland barley sheath rot is a panicle disease caused by Dactylobotrys gramincola, which seriously affects the yield and quality of highland barley. In this study, a set of specific primers were designed and screened based on the ITS sequence of D.gramincola based on loop-mediated isothermal amplification (LAMP). By optimizing the reaction temperature, a LAMP detection method with high sensitivity and rapid detection of D. gramincola was established. The specificity and sensitivity of the method were further evaluated, and the stem, flag leaf, and panicle tissues of the suspected diseased plants in the field were detected. The results showed that the LAMP detection system can effectively detect D. graminicola from six highland barley pathogens at 64 ℃ for 70 min. The detection sensitivity was 13.07 fg·μL-1, which was 100 times higher than that of the conventional PCR. The tissues of the suspected diseased plants in the field were detected. Ten positive samples were detected in the panicle; eight positive samples were detected in the flag leaf; and no positive samples were detected in the stem. The detection rate was 50%.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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