In order to explore the suitable nitrogen application rate for high-yield wheat in Huang-Huai-Hai region, the wheat variety Yannong 1212 was used as the test material. Four nitrogen application rates (0 kg·hm-2 (N0), 150 kg·hm-2 (N150), 210 kg·hm-2 (N210), and 270 kg·hm-2 (N270)) were set up in the field, and the effects of nitrogen application rate on the photosynthetic characteristics of flag leaves and grain filling of wheat under supplemental irrigation were analyzed. The results showed that the SPAD value of flag leaf at 14-28 days after anthesis under N210 treatment was significantly higher than that under N0 and N150 treatments, but not significantly different from that under N270 treatment. The photosynthetic gas parameters and photosynthetic enzyme activities of flag leaves under N210 treatment were significantly higher than those under N0 and N150 treatments at 14-28 days after anthesis, yet there was no significant change in photosynthetic parameters and photosynthetic enzyme activities of flag leaves when the nitrogen application rate increased to N270. The average grain filling rate and maximum grain filling rate under N210 treatment were significantly higher than those under other treatments, which was beneficial to the increase of grain weight. Compared with N0, N150 and N270, the 1 000-grain weight and grain yield under N210 treatment were the highest, and the yield increased 43.93%, 12.35% and 4.36%, respectively. In summary, the nitrogen application rate of 210 kg·hm-2 is the best nitrogen application rate for high yield under the conditions of this experiment.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |