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Abscisic acid (ABA) plays a crucial role in plant growth, development, senescence and stress response. The core signal transduction pathway is composed of PYL-PP2CA-SnRK2. In order to explore the protein functional characteristics, expression patterns and evolutionary relationships of PYL, PP2CA and SnRK2 in barley, the HvPYL, HvPP2CA and HvSnRK2 was identified from whole genome by bioinformatics methods. Gene structure and chromosomal localization, protein physicochemical properties, cis-acting elements in promoter region, expression analysis of different tissues and transcriptome expression patterns under salt stress were systematically analyzed. The results showed that 10 HvPYL, 13 HvPP2CA and 9 HvSnRK2 genes were identified from the barley genome. HvPYLs and HvPP2CAs were distributed in 1H-5H and 7H chromosomes, and HvSnRK2s were distributed in 1H-5H chromosomes. HvPYLs and HvPP2CAs were mainly located in chloroplasts, and HvSnRK2s are mainly localized in the cytoplasm. The motifs of the three family genes were highly conserved. The number of amino acids were less than 500, and the promoters contained a large number of plant response hormone elements. Genes of the three families were differentially expressed in roots, stems, inflorescences and seeds. HvPYL2 had the highest expression in root; HvPYL9 had the highest expression in episperm; HvPP2C-A1-10 had the highest expression in inflorescence. HvSnRK2.5, HvSnRK2.7, and HvSnRK2.8 had the highest expression in episperm. Transcriptome data analysis of a pair of near-isogenic lines with significant differences in salt tolerance at 48 h and 60 h under salt stress showed that HvPP2C-A2, HvPP2C-A4, HvPP2C-A6, HvPP2C-A9, HvPP2C-A10, and HvSnRK2.4 were significantly up-regulated by salt stress, while HvPYL6 and HvPYL7 were significantly down-regulated. The results of qPCR were consistent with the transcriptome data. The results showed that the PYL, PP2CA, and SnRK2 family played an important role in the response of barley to salt stress.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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