To explore the effects of sowing season and site on the antioxidant enzyme activity of barley, 48 barley varieties from Yunnan were sown continuously for two years in Dianyuan town during the four different seasons of spring, summer, autumn, and winter, and also sown at three different altitudes in Dianyuan, Xiaojie, and Yanhe town. The antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), and polyphenol oxidase (PPO) in barley grass powder were determined by microplate reader and differential analysis was conducted. The results showed that there were significant differences in the antioxidant enzyme activity of barley planted in different seasons and at different sites. Under the conditions of four-season planting, the SOD and CAT activities of summer-planted barley grass powder were significantly higher than those of spring, autumn, and winter planting, while the PPO activity of summer planting was slightly lower than that of autumn planting, but higher than that of spring and winter planting. The SOD and CAT activities of barley grass powder at different sites were all highest at the low altitude of Yanhe town but lowest at the high altitude of Dianyuan town. The PPO activity was highest at Xiaojie town but lowest at Yanhe town. The antioxidant enzyme activity of barley grass powder was mainly affected by the planting season. Combining the membership function method and cluster analysis, the antioxidant activity of barley was evaluated, and high-quality barley varieties with high antioxidant enzyme activities were preliminarily screened (Yunpi 22, Aisi 500, Yunke 1, Yunsimai 7).
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |