The development of high-quality salt-tolerant wheat varieties represents a significant trend in the advancement of agricultural development in China. In this study, Dekang 961 was used as the control, and 156 high-generation wheat breeding lines and three parental materials were subjected to different concentrations (0, 75, and 150 mmol·L-1) of NaCl stress treatments using the hydroponic method during the seedling stage. Meanwhile, high-quality specific molecular markers were used for assisted selection, combined with quality trait detection and analysis, to obtain wheat lines with strong salt tolerance at the seedling stage and excellent quality traits. The results showed that seven highly salt-tolerant materials, 42 salt-tolerant materials, 51 moderately salt-tolerant materials, 24 salt-sensitive materials, and 35 highly salt-sensitive materials were screened through cluster analysis of the comprehensive evaluation value D. Quality molecular marker identification revealed that 109 materials lack Sec-1 locus but possess the 5+10 subunit; five materials lack both the Sec-1 and Gli-D2 loci; three materials lack the Gli-D2 locus but possess the 5+10 subunit, and three materials lack both the Sec-1 and Gli-D2 loci while possess the 5+10 subunit. Comprehensive analysis led to the selection of one line with high salt tolerance and the pyramiding of three high-quality gene loci, three lines with high salt tolerance and the pyramiding of two high-quality gene loci, and three lines with high salt tolerance and the absence of the Sec-1 locus.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |