Two-line hybrid wheat is currently the main type of hybrid wheat in China. To analyze the genetic diversity and relationships between the parental lines of the two-line hybrid wheat, this study used the wheat 90K SNP (single nucleotide polymorphism) array to conduct a genome-wide screening of 123 parental lines of two-line hybrid wheat, and to estimate the genetic distance between lines and reveal their genetic diversity. The results showed that the polymorphism rate of SNP loci on the 21 wheat chromosomes reached up to 82.40%. The distribution of polymorphic loci on each chromosome ranged from 895 to 6 165, and the distribution of polymorphic markers among sub-genomes ranked as B>A>D, with the number of polymorphic markers distributed in 7 homoeologous groups in the order of 5>3>1>2>7>6>4. The genetic distance between the 123 hybrid wheat parental lines ranged from 0.001 to 0.513, with an average of 0.369. Among them, the genetic distance of the 60 male sterile lines ranged from 0.001 to 0.499, with an average of 0.324; the genetic distance of the 63 restorer lines ranged from 0.002 to 0.513, with an average of 0.372; and the genetic distance between the 60 male sterile lines with the 63 restorer lines ranged from 0.225 to 0.511, with an average of 0.387. The clustering results showed that all materials were divided into four different clusters. Integrated analysis of SNP and material types showed that the genetic distance between male sterile lines and restorer lines was the largest, followed by the difference between restorer lines, while the difference between male sterile lines was the smallest. Based on the analysis of genetic distance and relationships, it was found that the genetic differences among the two-line hybrid wheat parents are generally small, with some parents showing inbreeding tendencies. It is necessary to broaden the genetic background and increase the genetic diversity of the parents to improve the breeding level of hybrid wheat.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |