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The YABBY transcription factor is a type of plant specific transcription factor that plays an important role in leaf growth and floral organogenesis. It has a C2C2 zinc finger domain at the N-terminus and a spiral ring spiral YABBY domain at the C-terminus. This study used a spring wheat variety Xinchun 9 as the material and cloned a TaDL1 gene from the YABBY family, with a total coding region of 603 bp, encoding 201 amino acids. According to bioinformatics analysis, the molecular weight of TaDL1 protein was 22 765.21 Da, with an isoelectric point of 8.85, indicating an unstable hydrophilic protein. Through phylogenetic tree and conserved domain analysis, it was found that this gene belonged to the CRC subfamily of the YABBY family and was closely related to the rice OsDL gene. Through transient expression analysis of tobacco, TaDL1 protein was localized in the nucleus. Real time fluorescence quantitative analysis found that TaDL1 was specifically expressed in wheat young ears and leaves, with the highest expression level in leaves and second in young ears. TaDL1 responded to salt and heat stresses, with the most significant response to salt stress and sustained high expression levels within 2-24 h after stress. It is speculated that this gene may be involved in signaling pathways related to salt stress response.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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