Full
Outline
To investigate the differential expression genes (DEGs) related to angiogenesis in rosacea (RA) by utilizing bioinformatics analysis in order to screen the key genes and verify their mRNA expression levels.
The gene microarray dataset GSE65914 was retrieved from the Gene Expression Omnibus (GEO) repository. Analyzed by R programming, the dataset was refined to identify DEGs related to RA, and then cross-referenced with angiogenesis-related genes from the GeneCards database to get a subset specific to RA angiogenesis. The process of identifying key genes was augmented by employing protein-protein interaction (PPI) network analysis and Cytoscape-based computational algorithms. The mRNA expression levels of the aforementioned pivotal genes were detected by real-time fluorescent quantitative reverse transcription PCR (RT-qPCR).
A total of 947 RA-associated DEGs were identified from GEO dataset, and then 202 genes related to RA angiogenesis were further delineated. PPI network analysis and Cytoscape algorithm finally identified 3 key genes, that is, CXCL8, IL-1B, and STAT1. The results of RT-qPCR showed that the mRNA expression levels of MIP-2, GCP-2, IL-1B and STAT1 in RA lesions were significantly higher than those in normal controls (P<0.05).
With aid of bioinformatics analysis, our study has screened and validated key genes associated with angiogenesis in RA, namely CXCL8, IL-1B, and STAT1, which providing a theoretical basis for elucidating the potential mechanisms underlying RA-induced angiogenesis and developing targeted therapeutic strategies.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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