Full
Outline
To investigate the role and mechanism of CD4⁺ T-cell-derived IFN-γ in host defense against blood-stage Plasmodium berghei (P. berghei) infection.
A tamoxifen-inducible conditional knockout strategy was performed to generate mice lacking IFN-γ specifically in CD4⁺T cells (Ifngfl/flCD4-CreERT2). Then after the mice were infected intravenously with luciferase-expressing P. berghei ANKA (P. berghei ANKA-luciferase) strain, parasite burden, survival, and intra-erythrocytic developmental rhythm were monitored dynamically. Flow cytometry and ELISA were carried out respectively to quantify the splenic myeloid subsets and the titers of P. berghei-specific IgG and IgG2c.
CD4⁺T-cell-specific IFN-γ deficiency significantly reduced peripheral parasitemia on day 5 post-infection (P<0.05), showing no effect on survival or 24-hour intra-erythrocytic cycle of the parasite. Ifngfl/fl CD4-CreERT2 mice exhibited obviously decreased P. berghei-specific IgG2c (P<0.01) but increased total IgG (P<0.05). Frequencies and absolute numbers of neutrophils, monocytes, and macrophages remained unchanged.
During the process of P. berghei infection, IFN-γ secreted by CD4⁺T cells may decrease total serum IgG level by promoting class switching to IgG2c, and then enhance parasite burden after blood-stage P. berghei infection.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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