Full
Outline
To investigate the protective effects of mitochondrial preconditioning in pericytes (PC) against pulmonary vascular leakage in septic rats.
① 128 specific-pathogen-free SD rats (equal gender, 200±20 g) were randomized into: Sham group (postoperative tail vein injection of 0.5 mL saline), Sepsis (Sep) group (CLP+saline), PC group (CLP+untreated PC: 10⁶ cells/rat), and Mito-PC group (CLP+PC preconditioned with 2 μg mitochondria/10⁴ cells for 12 h). Assessments included: PC lung colonization (flow cytometry), pulmonary barrier function (Evans blue assay), lung histopathology (HE staining), serum organ injury markers [cTnT (cardiac), urea/creatinine (renal)], and inflammatory cytokines (TNF-α, IL-6). ② MSC-derived mitochondria were validated by electron microscopy/flow cytometry; primary retinal PCs from weaned SD rats were purity-verified (confocal microscopy). In vitro groups: Control (PC), Mito-PC, PC+H₂O₂ (0.5 μmol/L, 4 h), and Mito-PC+H₂O₂. Antioxidant capacity was assessed via pentose phosphate pathway (PPP) activity, NADPH levels, G6PD activity, and NADP⁺/NADPH ratio.
① Compared with Sham, Sep group showed significant increase in pulmonary leakage (Evans blue P<0.05), severe lung injury, elevated serum markers (TNF-α, IL-6, cTnT, urea, creatinine all P<0.05), 0% 72 h survival. PC group showed partial improvement (P<0.05). Mito-PC group demonstrated significant reduction in vascular leakage (P<0.05 vs PC group), improved histopathology and organ function (P<0.05), attenuated inflammation (P<0.05), higher 72 h survival rate (P<0.05).② Mitochondrial preconditioning significantly enhanced PPP activation and NADPH-mediated antioxidative capacity, Mito-PC+H₂O₂ vs PC+H₂O₂ showed improved cell viability (P<0.05), Mito-PC vs PC showed increased G6PD activity (P<0.05), decreased NADP⁺/NADPH ratio (P<0.05).
Mitochondrial preconditioning potentiates pericyte-mediated protection against sepsis-induced pulmonary vascular leakage through enhanced pentose phosphate pathway activity. Mitochondrial preconditioning potentiates pericyte-mediated protection against sepsis-induced pulmonary vascular leakage through enhanced pentose phosphate pathway activity.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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