Full
Outline
To investigate the effects of cinnamic aldehyde (CA) on Bcl-2-associated X protein (Bax) and Bcl-2 homologous antagonist/killer (Bak) in vascular endothelial cells of diabetic ulcer wound tissues, as well as on cell apoptosis.
① Forty-eight healthy SPF-grade male SD rats (5 weeks old, weighing 180~220 g) were randomly assigned to a control group (12 rats) and a diabetes group (36 rats). The diabetic model was established with an intraperitoneal injection of 50 mg/kg STZ-citrate sodium solution and high-fat diet feeding. The diabetes group was further randomly divided into Model group, CA group, and the rb-bFGF group, with 12 animals in each group. Wounds in the Con and Model groups were disinfected and topically treated with normal saline, CA group received topical application of 4 µmol/L CA in PEG 400 gel, and those of the rb-bFGF group were treated with bevacizumab gel. The wound healing rate of each group was calculated at 3, 7 and 14 d after intervention. At 14 d after intervention, pathological changes in the wounds were observed with HE staining, and the expression levels of Bax and Bak were detected by Western blotting. ② Human umbilical vein endothelial cell line EA.hy926 was treated with 175 mmol/L glucose for 48 h to establish a cell model of high glucose injury. The experimental cells were divided into control group, model group and CA treatment group. Cell scratch test and tube formation test were performed respectively to determine the migration ability and angiogenesis of the cells. The expression levels of Bax and Bak was detected with immunofluorescence assay, and cell apoptosis was detected by TUNEL staining.
①The diabetic rats in the Model group exhibited significantly higher blood glucose level (P<0.05), declined wound healing rate at 7 and 14 d after intervention (P<0.05), and enhanced expression levels of Bax and Bak (P<0.05) when compared with the control group. Pathological observation revealed that, at 14 d after intervention, accompanied with inflammatory reactions, dense infiltration of inflammatory cells, fewer new blood vessels, and continuous fluid exudation in the wound were observed in the Model group, but the control group presented complete epithelialization in full-thickness skin. Compared with the conditions in the Model group, both CA and rb-bFGF treatment improved the epithelialization process, with mature granulation tissues, showing good healing condition, promoted wound healing rate (P<0.05), and decreased the expression levels of Bax and Bak (P<0.05). ② The results of cell experiments showed that the cells of the model group showed significantly reduced migration ability and tube formation ability (P<0.05), reduced protein levels of Bax and Bak (P<0.05), and lower apoptotic rate (P<0.05) when compared with the cells in the model group.
CA can inhibit the expression of apoptosis-related proteins Bax and Bak, promote the migration and tube formation of vascular endothelial cells, and inhibit the cell apoptosis under high glucose condition, which may be an important reason for its promoting wound healing in diabetic ulcer rats.
25
10
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
