Full
Outline
To investigate the effect of β-arrestin1 (ARRB1) on cell proliferation, migration and invasion in oral squamous cell carcinoma (OSCC).
Based on The Cancer Genome Atlas (TCGA) database, the expression profiles of ARRB1 in OSCC were analyzed, and then Gene Set Enrichment Analysis (GSEA) was used to suggest the possible signaling pathways involved, and to explore its potential impact on the prognosis of OSCC patients. Immuinohistochemistry (IHC) was performed to detect the expression of ARRB1 in OSCC tumor tissues and adjacent tissues, and the correlation between ARRB1 expression and clinicopathological features was statistically analyzed. The expression profiles of ARRB1 in SCC-15, CAL-27 and HOK cell lines were verified by qPCR and Western blotting. The ARRB1 overexpression plasmid model was constructed, and its effects on the proliferation, migration and invasion of OSCC cells were analyzed by clone formation, EdU, scratch and Transwell assays.
TCGA showed that the expression level of ARRB1 was significantly lower in head and neck squamous cell carcinoma (HNSC) and OSCC tissues than the corresponding normal tissues (P<0.01). The expression of ARRB1 in OSCC tissues was correlated with tumor differentiation, lymph node metastasis and TNM stage (P <0.05). The OSCC patients with high expression of ARRB1 had a lower survival rate than those with low expression (P<0.01), which was consistent with the results of bioinformatics analysis. The expression level of ARRB1 in SCC-15 and CAL-27 cells was lower than that of HOK cells (P<0.01), and its overexpression significantly inhibited cell proliferation (P<0.05), migration (P<0.01) and invasion (P<0.01).
ARRB1 is lowly expressed in OSCC, its overexpression inhibits the proliferation, migration and invasion of OSCC cells, and it is related to prognosis improvement.
34
13
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
