Full
Outline
To investigate the effects of open reading frame 1 protein (ORF1p), encoded by long interspersed nuclear element-1 (LINE-1), on the proliferation, migration, and invasion of esophageal squamous cell carcinoma (ESCC) cells, and explore the underlying molecular mechanism.
① Western blotting was performed to compare the expression of ORF1p between normal esophageal squamous epithelial cells and ESCC cells. ② Immunohistochemistry (IHC) assay was used to examine ORF1p expression in ESCC tissues and paired normal tissues adjacent to tumor. ③ The effects of ORF1p knockdown and overexpression on malignant behaviors in ESCC cells were determined through functional assays. ④ Xenograft tumor model in nude mice was established to evaluate the impact of ORF1p on tumor growth in vivo. ⑤ Transcriptome sequencing combined with cell functional rescue experiments were conducted to identify downstream targets regulated by ORF1p.
① Western blot analysis demonstrated the expression of ORF1p was significantly higher in the ESCC cell lines than the normal esophageal squamous epithelial cells (P<0.05). ② IHC confirmed remarkable up-regulation of ORF1p in ESCC tissues than paired adjacent normal tissues (P<0.000 1). ③ Functional assays and experiments on xenograft tumor models revealed that ORF1p substantially enhanced the proliferation, migration, and invasion of ESCC cells, as well as tumorigenic potential in vivo (P<0.05). ④ Functional rescue experiments showed that ORF1p facilitated the proliferation, migration, and invasion of ESCC cells by modulating AJUBA expression (P<0.05).
ORF1p is significantly up-regulated in ESCC and promotes the proliferation, migration, and invasion of ESCC cells by regulating AJUBA expression.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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