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To address the issues of restricted hydrolysis rate and low methane production in the anaerobic digestion of agricultural waste, zerovalent iron(ZVI) with different doses(4,8,12 mg/L) and particle size(microscale, nanoscale) was added during digestion after thermal pretreatment to investigate its enhancement of the codigestion of cow manure and corn straw. The research indicated that the appropriate addition of ZVI promoted both the hydrolysis acidification and methane production processes. The maximum cumulative methane yield was achieved with the addition of 8 g/L microscale ZVI or 4 g/L nanoscale ZVI, which increased by 20.7% and 29.5%, respectively compared with the control group. Microscale and nanoscale ZVI facilitated the release of dissolved organic compounds and the conversion of propionic acid to acetic acid. Nanoscale ZVI exhibited a stronger enhancement effect on hydrolysis and acidification than micronscale ZVI. However, excessive doses (8,12 g/L) of nanoscale ZVI had an adverse effect on methane production. ZVI promoted the enrichment of hydrolytic acidogenic bacteria and acetotrophic methanogens, such as Romboutsia, Saccharofermentans and Methanothrix, which enhanced the processes of hydrolysisacidification and methanogenesis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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