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In this paper, Miscanthus lutarioriparius (ML) cellulose was used as the raw material to produce bioethanol by a fedbatch synchronous saccharification and fermentation process. The effects of initial substrate concentration, final substrate concentration, and feeding times on ethanol yield were analyzed. The orthogonal experiments were used to optimize the process, and the optimal process parameters were obtained as follows: Initial substrate concentration was 17.5%, and the batch feeding was conducted twice until the final substrate concentration was 32.5%. Under these conditions, the highest ethanol content could reach to 70.15 g/L, which increased by 23.36% compared to the synchronous saccharification and fermentation process without fedbatch. The material balance analysis was conducted on the basis of the optimal process. The structure of ML cellulose before and after fermentation was characterized by scanning electron microscopy, fourier transform infrared spectroscopy, and Xray diffraction. The results showed that the enzymatic saccharification and fermentation process would change the internal structure of ML cellulose, break the hydrogen bond or covalent bond, and increase the crystallinity of the substrate.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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