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In this paper, biochar composite carrier was constructed by using biochar loaded with different concentrations of sodium bicarbonate, humic acid, Tween 20 and other catalytic regulators, and each composite carrier was added to the rice stubpig manure mixed anaerobic fermentation system for anaerobic fermentation. By measuring the gas production, methane production and the degradation rate of straw lignocellulose of each treatment, the role of biochar composite carrier on methane production in mixed anaerobic fermentation and straw degradation was revealed. The results show that the biochar composite carrier can increase the peak value of methane production and shorten the time of peak value of methane production. Biochar composite carriers can significantly increase the cumulative gas production and the cumulative methane production of anaerobic fermentation systems, with the best effect of biocharloaded Tween 20, followed by biocharloaded humic acid and biocharloaded sodium bicarbonate. Compared with the control group, the gas production and the methane production of biochar loaded Tween 20 treatment increased by 23.18% and 62.20%, respectively. In each treatment, the degradation degree of straw was the highest in biochar loaded Tween 20 treatment, followed by biocharloaded humic acid, biochar loaded sodium bicarbonate and control group. The optimal loading concentrations of Tween 20, humic acid and sodium bicarbonate were 2.25, 0.75, 2.10 g/L, respectively.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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