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The study elucidated the relationship between anaerobic digestion gas production efficiency and temperature and hydraulic retention time (HRT) using synthetic glucose wastewater as a substrate. Gas production under different temperatures (37,55 °C) and HRTs (25, 30, 50 d) was compared. The results indicated that the hydrolysis rate of glucose was higher at thermophilic temperature than at mesophilic temperature. However, volatile fatty acids, especially propionic acid, tended to accumulate at thermophilic temperature. Additionally, Methanomicrobiaes and Methanosarcinales were enriched at both moderate and high temperatures, suggesting the presence of pathways for methane production from acetic acid and acetate oxidation at both temperatures, with the acetate oxidation pathway exhibiting greater environmental resilience. The recommended optimal fermentation conditions for treating heavy glucosecontaining wastewater through anaerobic digestion are 37 °C and an HRT of 30 days.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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