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Research on enzymatic hydrolysis and saccharification process of corn straw based on mild pretreatment
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Huanhuan Zhang1, Yucheng Lin1, Yuanyuan Wang1, Shiqiang Zhao1, Chaojun Du2, Zigao Zhao3, Chun Chang1, 4
Renewable Energy Resources | 2025, 43(2) : 143 - 151
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Renewable Energy Resources | 2025, 43(2): 143-151
Research on enzymatic hydrolysis and saccharification process of corn straw based on mild pretreatment
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Huanhuan Zhang1, Yucheng Lin1, Yuanyuan Wang1, Shiqiang Zhao1, Chaojun Du2, Zigao Zhao3, Chun Chang1, 4
Affiliations
  • 1 School of Chemical Engineering Zhengzhou University Zhengzhou 450001 China
  • 2 Nanyang Research Institute of Zhengzhou University Nanyang Institute of Technology Nanyang 473004 China
  • 3 National Key Laboratory of Biobased Transport Fuel Technology Nanyang 473000 China
  • 4 Henan Center for Outstanding Overseas Scientists Zhengzhou 450001 China
Published: 2025-02-20
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Pretreatment and enzymatic hydrolysis of straw type biomass are among the key technologies for its high value conversion and utilization. Using corn stover as the raw material, pretreatment methods with formic acid, sodium chlorite, and alkaline hydrogen peroxide were studied and compared. Methods such as scanning electron microscopy, Xray diffraction, and Fouriertransform infrared spectroscopy were employed to analyze the composition, morphology, crystal structure, and functional groups of corn straw before and after pretreatment. After pretreatment with formic acid, sodium chlorite, and alkaline hydrogen peroxide, the lignin removal rates were 66.71%, 97.12%, and 91.88% respectively, and the enzymatic hydrolysis rates reached 63.26%, 71.83%, and 95.14% respectively. Under the conditions where the cellulase dosage is 19.6 FPU/g, the xylanase dosage is 35.44 IU/g, and the Tween 80 dosage is 20.96 mg/g, the predicted enzymatic hydrolysis rate of corn straw pretreated with alkaline hydrogen peroxide is 95.57%, while the actual enzymatic hydrolysis rate is 94.42%. The optimal feeding strategy for high solid enzymatic hydrolysis was an initial substrate concentration of 8%, with 4% of the substrate added at 6, 12 h, and 24 h respectively. After 120 h of enzymatic hydrolysis, the enzymatic hydrolysis rate reached 87.57%.

corn stover  /  pretreatment  /  high solid enzymatic hydrolysis  /  fed-batch
Huanhuan Zhang, Yucheng Lin, Yuanyuan Wang, Shiqiang Zhao, Chaojun Du, Zigao Zhao, Chun Chang. Research on enzymatic hydrolysis and saccharification process of corn straw based on mild pretreatment[J]. Renewable Energy Resources, 2025 , 43 (2) : 143 -151 .
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Year 2025 volume 43 Issue 2
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  • Receive Date：2023-08-19
  • Online Date：2025-07-18
  • Published：2025-02-20
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  • Received：2023-08-19
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    1 School of Chemical Engineering Zhengzhou University Zhengzhou 450001 China
    2 Nanyang Research Institute of Zhengzhou University Nanyang Institute of Technology Nanyang 473004 China
    3 National Key Laboratory of Biobased Transport Fuel Technology Nanyang 473000 China
    4 Henan Center for Outstanding Overseas Scientists Zhengzhou 450001 China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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