To achieve efficient advanced nitrogen and phosphorus removal from wastewater while simultaneously recovering energy, denitrifying phosphorus removal and electrogenesis (DPRE) system was constructed to research the influence of mass concentration of organic substance on nitrogen and phosphorus removal and electricitygenerating performance using synthetic domestic wastewater. The results showed that the influent mass concentration of organic substance had a minor effect on COD removal but significantly influenced nitrogen and phosphorus removal and power generation. When the influent COD concentration was 200~300 mg/L, the DPRE system achieved optimal pollutant removal, with the removal rates of COD, NH4+N and PO43P reaching 86.07% ~86.22%, 83.94%~85.10% and 80.29%~83.38%, respectively. When the influent COD concentration was 300~400 mg/L, the system exhibited the best electricitygenerating performance, with an average power density of 36.49~39.47 mW/m². When the influent COD concentration was 300 mg/L, the DPRE system could simultaneously obtain highefficiency removal of nitrogen and phosphorus and electricity generation.