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Outline
In order to reduce the cost of microbially induced carbonate precipitation (MICP), the effects of industrial-grade bovine bone protein peptone dosages, soya peptone dosages, sodium chloride dosages and urea dosages on the microbial quantity OD600 value and urease activity of Sporosarcina pasteurii cultured for 16 hours were studied. Response surface methodology was used to optimize the optimal ratio of industrial culture medium. And the microbial quantity OD600 value and urease activity of the bacterial solution under the optimal ratio were detected. After the bacterial solution cultured for 16 hours reacting with different concentrations of cementing solution, the amount of calcium carbonate produced by the two reactions was measured, and the calcium carbonate crystals was analyzed using scanning electron microscopy (SEM). The results show that the optimal medium ratio is 25 g/L of bovine bone protein peptone, 7.5 g/L of soya peptone, 5 g/L of industrial sodium chloride, 22.2 g/L of industrial urea, and 1 000 mL of pure water. The OD600 value of Sporosarcina pasteurii liquid cultured for 16 hours under this ratio is 1.672, and the urease activity is 0.037 mmol/(L·min), both of which are within the prediction error, indicating feasibility of the results. The content of calcium carbonate generated by cementation under the two media is not much different, and the cementation effect of industrial-grade culture medium bacterial solution is stronger than that of analytical pure culture medium bacterial solution. The research results can provide a low-cost medium ratio scheme for the large-scale engineering application of MICP.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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