In order to realize the safe and efficient mining of isolated pillars in metal mines transitioning from open-pit to underground mining, Mathews stability diagram method was used to calculate stope parameters. Combined with FLAC3D software, the influence of different mining methods on stope stability was analyzed. Upward drift filling mining method + construction of artificial false roof mining scheme was verified by field test. The results show that the hydraulic radius of the hanging wall of the designed stope is 1.64 m, and the maximum span of the roof without support is 3.5 m, both of which are within the safe range of theoretical calculation. The structural parameters of the stope meet the safety requirements. Under the mining method of skip mining, the maximum compressive stress and maximum tensile stress of the stope are less than the compressive strength and tensile strength of the rock mass, and the formed plastic zone does not penetrate the stope, indicating that the stope is in a stable state. In the field test, the production capacity of a single ore block reaches 100 t/d, the loss rate is 4%, and the dilution rate is 5% after the application of the mining scheme of the upward drift filling mining method + 1.5 m thick reinforced concrete artificial false roof and the mining method of skip mining. The number of ore blocks is stable and the economic benefit is good.