Construction of science popularization capacity and science popularization resources not only requires construction of “hard resources” such as infrastructure in science and technology museum and science popularization exhibits, but also requires construction and improvement of “soft resources” such as professionals of science and technology museum. The quality of professional team directly decides the comprehensive strength of science and technology museum, capacity to design science popularization exhibits and level of scheming and organizing science popularization activities and carrying out science popularization services. With rapidly developing high technology, it is imperative for sustainable development of science and technology museum undertaking in China to forge a professional team of science and technology museum with appropriate scale, reasonable structure, excellent quality and powerful guarantee. This paper takes China Science and Technology Museum as an example, proposes existing deficiencies in construction of professional team of China Science and Technology Museum through analyzing current situation of professional team of China Science and Technology Museum, presents strategic management concept for professional team of China Science and Technology Museum through SWOT model and puts forward suggestions and imaginations of corresponding measures targeted at existing problems.